Coding

Part:BBa_K125500:Design

Designed by: Grace Kwan   Group: iGEM08_Hawaii   (2008-07-25)

GFP fusion brick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

Two nucleotides (T and G) of the GFP start codon were removed to create this fusion brick via site-directed mutagenesis with VR and the following primer:

Primer name Primer Length G/C content Tm
GFPE0040_forward GCCGCTTCTAGAcgtaaaggag 22 bp 54.55% 60.2 C

Source

The GFP fusion brick was derived from the GFP BioBrick, BBa_E0040.

References